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Print near letter-quality multi-font documents and replace supplies less often. Powerful printheads last up to 300 million characters and re-inking ribbons that print up to 8 million characters are available.

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The problem is that your string is not just '1151226468812.22', but it is '"1151226468812.22"'. It also contains speech marks ("). This means that before you convert this to a float, you need to remove the leading and trailing speech marks. Luckily, Python has a very handy string method .strip() to do this for you.

CAMWorks® with Machining Intelligence is the most advanced CAM programming software available for getting products to market faster, more efficiently and within budget. CAMWorks is a next generation best-in-class CNC programming solution that enables users to program smarter and machine faster. View more

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With its unique property to efficiently condense several plasmids of different sizes, PEIpro® transfection reagent is ideal for optimal co-transfection of several plasmids containing the gene of interest and necessary viral components to produce full recombinant virions. PEIpro® has benefited from extensive developments that made it the unique PEI-based transfection reagent that can offer the flexibility and robustness that is needed during Process Development and Scale-up; it is compatible with commercially available adherent and suspension virus production systems for lentivirus and AAV viral vectors(Table 1). Published work on AAV and lentivirus production with PEIpro®, and on other types of viruses (adenovirus, retrovirus virus-like particle (VLP)) production, are available under the Bibliography tab.

Suspension cell culture-based systems can offer considerable advantages in simplifying the scale-up process, in terms of cell culture conditions and harvesting. For this several animal-free synthetic media are commercially available to maintain high productivity in cells lines adapted for growth in suspension. Depending on the type of viral vector produced in suspension cell systems, the transfection reagent is critical to reach high viral vector yields. For lentivirus viral vectors produced in suspension cell sytems, high virus production yields can be reached by transfecting suspension-adapted HEK-293T cells with PEIpro® in BalanCD® HEK293 (Irvine Scientific) (Fig. 1).

NEW! FectoVIR®-AAV transfection reagent is a novel class of transfection reagent specifically designed to increase recombinant AAV production in suspension cell systems. The increased production yield is reproducible at different scales, for the production of several AAV serotypes (Read more on FectoVIR®-AAV).Fixed-bed bioreactors are also an attractive scale-up model as they allow for increased volumetric productivity with low shear stress cultivation of adherent cells. Development of scalable viral vector production has been successfully implemented in the fixed-bed reactor iCELLis® (Pall) in combination with the use of PEIpro®. For example, during the scale-up production of a recombinant AAV-8 (rAAV-8) from 0.8 m2 to 4 m2 iCELLis® Nano bioreactor, the established PEIpro® transfection protocol could be readily transferred without further optimization. The virus yields obtained in the 4 m2 iCELLis® Nano bioreactor demonstrated the process scalability and robustness. For more details, see here.

The extensive research development invested in PEIpro® and the manufacturing process developed by Polyplus-transfection® has made it the unique PEI-based transfection reagent that benefits from extensive research development in polymer chemistry and formulation, in terms of polymer structure, deacetylation degree, polydispersity index and molecular weight. As a result, PEIpro® is the gold reference among commercially available PEIs and requires lowest DNA amount and volume of reagent to ensure reproducibly high production yields (Fig. 2).

Calcium phosphate (CaPO4) is known to be inefficient for transfection of cells grown in suspension, costly in the amount of plasmid DNA required, and inconsistent due to several fluctuating parameters during complexation (pH, calcium and phosphate concentrations). Compared to CaPO4, PEIpro® is efficient with high transfection efficiencies ranging from 70 to 90% both in adherent and in suspension cells, which correspondingly leads to higher virus titer yields (Fig. 3).

PEIpro® is manufactured and formulated using a highly controlled and strict manufacturing process. By ensuring reproducibility from one run to the next, irrespective of the operator, PEIpro® greatly facilitates process standardization; a key advantage for large-scale production. Moreover, PEIpro® is released using advanced quality controls including a specification for transfection efficiency that guarantees excellent lot-to-lot consistency (Fig. 4).

PEIpro® is fully synthetic and free of any animal-origin component. The quality of PEIpro® is continuously assessed and documented during manufacturing with the appropriate control testings. Further systematic lot management and release testing is performed for each lot produced. A qualified transfection assay ensures reliability and reproducibility of the transfection efficiency under conditions suitable for biomanufacturing. PEIpro® undergoes sterility, mycoplasma and bacterial endotoxin testing according to pharmacopoeia standards. A full list of QCs is available upon request.

In addition, Polyplus-transfection® is ISO 9001 Quality Management System accredited since 2002; this level of certification assures global customers that the supplier has established reliable and effective processes for product development, manufacturing, sales and customer support.

At PHW Services, we are fully committed to providing our customers with the highest quality of products and services so that they can enjoy a more comfortable and efficient home. Through our many years of experience, we offer prompt, efficient and friendly services to get you back into comfort in your own establishment. Give us a call today to schedule an appointment with our team now.

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Wrong data entry can be enough to steer your CNC machine into a crash. With ZOLLER's »zidCode« and »zidCode 4.0« solutions, you can transmit the measurement and setting data as well as the tool ID digitally and error-free to almost any common CNC machine and thus avoid the machine crash.

»zidCode« quickly gets your production ready to use digital tool data conveniently in your processes. After just a short time, your data will flow to the CNC machine without errors and in a control-ready manner.

The »zidCode 4.0« communication platform not only ensures correct and secure data transfer of the tool geometry data, but also speeds up the tooling system in the various machines enormously. Once you have completely created the tool data, you can also transfer all machine-relevant tool data to any machine control via scanning.

You remove the tool holder, which is provided with a 2D code, mount the cutting tool and you're ready to go. By simply scanning the code, the data is immediately recorded and inventoried in the »pilot« software and is available for further processing.

After the 2D code of the complete tool has been scanned and the tool has been inserted, everything else takes place quasi automatically, because: Thanks to this networked solution, the ZOLLER setting and measuring device knows exactly which measuring programme is intended for this particular tool. After the measurement, the data is stored in the software or the central tool database z.One.

Scan tools at the »zidCode« unit and your machine automatically receives the corresponding tool data from the network. Thanks to the post-processors and the data prepared in accordance with the control system, your machine can start immediately and produce only correct components. 2b1af7f3a8