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Familiarity is an important thing when it comes to fashion. Therefore, we thought we would start off with the OG legwarmers that have been a constant for generations. Once we introduce the major leg warmers, or tights, trends of the season, we'll unveil our favorite tights brands, the best accessories to match your favorite legwarmers, and other news that we think you'll find interesting. For the 2018 holiday season, prepare for news you can't help but appreciate.A calcium-binding photoprotein tethered to DNA in vitro generates a change in fluorescence intensity over time.
A calcium-binding photoprotein (aequorin) was inserted into a plasmid DNA. The resultant construct could be transfected into MOLT-4 (a human T-cell line) cells without selective death. The plasmid DNA, however, was unable to induce the photoprotein in the transfected cells. This is probably due to the interaction of the Ca2+-binding photoprotein with the nucleic acid. Aequorin, of molecular weight 14,800, was also tethered to a semi-synthetic DNA; the construct was also unable to induce aequorin in MOLT-4 cells. On the other hand, the G-rich synthetic DNA was able to trigger the Ca2+-bound photoprotein. The calcium-binding photoprotein (apoaequorin) was able to produce a time-dependent change in fluorescence intensity upon light irradiation. This confirmed that photoprotein anchored to the nucleic acid is retained in the cells and is functional for the detection of Ca2+.A Muslim has told how she was so concerned about the cash she received from her church for food she was trying to cover up the money that she distributed it to her family instead.
Today's Daily Mail, reports that a Muslim foodbank volunteer, Shabir Zeeshan, explained how she thought it would be "unfair" to give food aid to the richer congregation at her church, which happens to be the Baptist Church of Strathfield, Sydney, Australia.
The centre she worked for the Grace Food Bank distributes food to poor and homeless people.
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